Fungi - Candida albicans - Research News, Data, Publications & Aproaches - ERG11 Mutations - Telomeres - Sub-Telomeric Structures - Nuclear Biology & Nuclear Chemistry Aproaches - Structural Fragility and Replication Dynamics Near the ERG11 Subtelomeric Region in Candida albicans - Non-Elaborate Posts - Post 2

 The telomeric neighborhood of ERG11 is intrinsically prone to double-strand breaks (DSBs) due to replication stress, oxidative assault, and the repetitive nature of terminal DNA sequences (Anderson et al., 2015). These DSBs serve as initiation sites for repair pathways, yet their repair mode is profoundly influenced by local chromatin states. In compact heterochromatin, the accessibility of HR machinery is limited, favoring alternative pathways such as non-homologous end joining (NHEJ) and microhomology-mediated end joining (MMEJ). These mechanisms, inherently error-prone, introduce insertions, deletions, and micro-rearrangements that subtly alter ERG11’s coding frame and regulatory elements. Thus, the subtelomeric structure itself acts as a molecular incubator for variation, sculpting mutability through architecture.

Comments

Post a Comment

Popular posts from this blog

   At the biochemical level, ERG11 ’s function as a heme-dependent monooxygenase interlocks with the cell’s oxidative balance. Heme fluctuations within the nucleus can alter the activity of heme-responsive transcription factors and chromatin modifiers, introducing a chemical feedback loop between metabolism and genetic variation (Puig & Gutiérrez, 2022). Reactive oxygen species generated by azole stress promote DNA oxidation and base substitution events preferentially within open chromatin domains. This coupling of redox chemistry with mutation formation constitutes a nuclear-scale biochemical evolution engine—one where chemical disequilibrium catalyzes genomic diversity in real time.
Read more

Fungi - Candida auris - Non-Elaborate Posts - Post 2

Genetics and genetic mutations for resistance to fluconazole ERG11 mutations (notably Y132F, K143R) are common contributors to fluconazole resistance; and Cas9 editing showed direct contribution of these alleles to elevated MICs. Additional determinants (TAC1B, efflux pumps, aneuploidy) amplify resistance. Different geographic clades show distinct ERG11 allele frequencies. Genome assemblies reveal structural variation and copy-number differences Resistance evolves under strong antifungal selection in hospitals. Surveillance sequences help track clonal spread and mutation patterns
Read more
In some settings, susceptible-dose dependent (SDD) categorization is used: isolates with intermediate MICs may still respond to higher fluconazole doses, but the presence of ERG11 mutations often reduces the likelihood of clinical success even with dose escalation. ( PMC ) Beyond single substitutions, clinical isolates frequently carry combinations of ERG11 changes plus efflux-regulatory mutations (e.g., TAC1 gain-of-function) that together produce higher MICs and poorer outcomes; thus, sequencing plus expression data can refine prognostic interpretation.
Read more