Fungi - Candida albicans - Research News, Data, Publications & Aproaches - ERG11 Mutations - Telomeres - Nuclear Biology & Nuclear Chemistry Aproaches - Nuclear Architecture - Spatial Reprogramming - Non-Elaborate Posts - Post 6

 The nuclear relocation of ERG11 does not occur in isolation; it is accompanied by topological remodeling of the local chromatin fiber. Fluconazole exposure promotes the formation of chromatin loops that bring distal regulatory sequences into proximity with the ERG11 promoter. This looping is facilitated by cohesin and condensin complexes, whose ATP-dependent activity is sensitive to nuclear redox balance. As chromatin loops form and dissolve, the ERG11 locus experiences changing transcription factor occupancy — particularly of Upc2p, the master sterol biosynthesis regulator. Thus, the mechanical act of looping generates a biochemical gradient of transcriptional accessibility, merging spatial and chemical regulation into one continuous system.

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