Fungi - Candida albicans - Research News, Data, Publications & Aproaches - ERG11 Mutations - Telomeres - Epigenetic Modulation and Histone Chemistry of the ERG11 Locus - Nuclear Biology & Nuclear Chemistry Aproaches - Non-Elaborate Posts - Post 7

 

 A striking feature of the ERG11 regulatory system is its sensitivity to the nuclear redox environment. The heme-dependent nature of lanosterol 14α-demethylase introduces a feedback mechanism wherein intracellular redox status modulates both enzymatic activity and chromatin accessibility. Reactive oxygen species generated during azole exposure alter the oxidation states of cysteine and lysine residues on histone-modifying enzymes, affecting their catalytic efficiency (Puig & Gutiérrez, 2022). The oxidative modification of Hda1p or Sir2p can therefore reprogram histone acetylation landscapes, linking chemical reactivity directly to gene expression control. This confluence of redox and epigenetic chemistry situates

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   At the biochemical level, ERG11 ’s function as a heme-dependent monooxygenase interlocks with the cell’s oxidative balance. Heme fluctuations within the nucleus can alter the activity of heme-responsive transcription factors and chromatin modifiers, introducing a chemical feedback loop between metabolism and genetic variation (Puig & Gutiérrez, 2022). Reactive oxygen species generated by azole stress promote DNA oxidation and base substitution events preferentially within open chromatin domains. This coupling of redox chemistry with mutation formation constitutes a nuclear-scale biochemical evolution engine—one where chemical disequilibrium catalyzes genomic diversity in real time.
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Fungi - Candida auris - Non-Elaborate Posts - Post 2

Genetics and genetic mutations for resistance to fluconazole ERG11 mutations (notably Y132F, K143R) are common contributors to fluconazole resistance; and Cas9 editing showed direct contribution of these alleles to elevated MICs. Additional determinants (TAC1B, efflux pumps, aneuploidy) amplify resistance. Different geographic clades show distinct ERG11 allele frequencies. Genome assemblies reveal structural variation and copy-number differences Resistance evolves under strong antifungal selection in hospitals. Surveillance sequences help track clonal spread and mutation patterns
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